The Center for Lignocellulose Structure and Formation (CLSF) is focused on developing a detailed understanding of lignocellulose, the main structural material in plants.

Perspective

Every living organism on Earth uses glucose as an energy source. Plants not only make glucose from sunlight, water, and carbon dioxide, but they convert much of it into an energy-rich material – the lignocellulosic cell wall – that is both a versatile material and a recalcitrant feedstock for liquid biofuel production, both properties stemming from its hierarchical structure at the nano- to mesoscales. Our research addresses key questions in plant biology: by understanding the fundamental science of how plants manufacture lignocellulose, we may devise new ways to control it (through genetic engineering) and transform it (through chemical engineering) for improved technologies to supply our energy and material needs for a sustainable future.

Research Plan and Direction

CLSF researchers investigate:

  • how plants transform glucose into the crystalline nano-fibril (cellulose) with very different physical properties from the starting material
  • how cellulose is combined with other polymers to make cell walls with diverse physical properties

These manufacturing processes, practiced by nearly every plant cell, greatly exceed the current capabilities of human technologies. The goals of the CLSF are to develop a detailed nano- to mesoscale understanding of plant cell walls, from glucose polymerization and glucan crystallization into cellulose microfibrils to the orderly hierarchical assembly of the components to form the mature plant cell wall. Success in our research goals will offer many lessons on how to create such hierarchical structures, how to vary and manipulate them, and potentially how to disassemble them more efficiently than is currently possible. Our team of accomplished biological, physical, and computational researchers with their diverse technical expertise and team collaboration work towards these goals which straddle biology and physics.

Research Highlights

  • CLSF researchers hypothesised that the side chains of the polysaccharide xylan – a major component of grass cell walls – is significantly affecting cell wall properties. The authors engineered the model grass Brachypodium distachyon by reducing xylan side chains and found that this led to a less tightly bound cell wall. A looser cell wall polymer network allows easier penetration of digestive enzymes that break down biomass. The findings published in New Phytologist (Tryfona et al., 2024), provide a better understanding of the molecular architecture of grass cell walls and open new avenues to improve cereals for food and to use grass biomass for animal feed, the production of bioenergy and biomaterials.

    Cellulose microfibrils depicted as fairly parallel gray tubes 7.4 nm apart interspersed with pectin as narrower strings

    Proposed arrangement of cellulose microfibrils (Yu & Del Mundo et al., 2024)

  • A CLSF collaborative team used a suite of characterization tools including atomic force microscopy and x-ray scattering to uncover the dynamic structural changes of primary cell walls under strain (Yu & Del Mundo, et al. 2024). Upon deformation, the walls undergo multiscale rearrangement, from mesoscale cellulose microfibril network realignment to atomistic scale crystalline cellulose backbone extension. These observations reveal the structural basis for the walls’ ability to withstand increasing stress during extensive deformations, providing insights into the structure-function relationship of cell walls and guiding the design of new biomimetic materials that are both strong and extensible.
  • A team of plant biologists and engineers used advanced X-ray techniques to discover distinctive calcium-pectin nanostructures within the cell walls of Arabidopsis thaliana plants. When these structures were perturbed, cellulose microfibrils were repositioned, indicating direct structural links between pectins and cellulose. This work, led by Oskar Siemianowski, Enrique Gomez and Charles T. Anderson and published in the Journal of the American Chemical Society Au, highlights the importance of pectins in maintaining the structural integrity of plant cell walls.
Colorful hybrid structural molecular model of both sides of GhCESA1

hybrid GhCESA1 model from Kwansa et al., 2024

  • Research showing the structural determination of a full-length plant cellulose synthase, Kwansa et al., 2024, was highlighted on the cover of the February 2024 issue of Cellulose.
  • “Most polysaccharide biosynthesis and some modifications, including methylation, occur in the Golgi body. Thus, identifying potential transporters of SAM into the Golgi is important for understanding the role of pectin modifications in cell wall structure and function.”   The editorial team of Nature Plants provided this statement in their research briefing “Shutting the door on polysaccharide methylation”  which highlighted CLSF’s latest research publication Golgi-localized putative S-Adenosyl methionine transporters required for plant cell wall polysaccharide methylation Temple et al., 2022. (June 2022)
  • A CLSF collaborative team used an innovative combination of cryo-electron tomography and sensitivity-enhanced solid-state NMR to detail the structure of cellulose fibers synthesized in-vitro by CESAs present in membrane fractions of Physcomitrium patens (a moss) in Deligey, Frank et al., 2022. The combination of these techniques allows us to detail both the nanoscale assembly and atomic-level structure of cellulose fibers. The work is published in Biomacromolecules with a highlight as a supplementary journal cover.   (April 2022)
  • Lignin structure and xylan conformation regulate the physical packing interactions of lignin and polysaccharides and have variable patterns among grasses, hardwoods, and softwoods.  These findings by Kirui et al. utilizing solid-state NMR, unveil the principles of polymer interactions underlying the heterogeneous architecture of lignocellulosic materials. The article is published in Nature Communications and is selected by the editors as a featured article within the topic of Structural Biology, Biochemistry and Biophysics.  (January 27, 2022)
  • The results of altering the protein sequence of a CESA gating loop, which is predicted to help regulate cellulose synthase enzyme activity, is reported in Burris et al. 2021.  Seven changes were tested in a secondary wall cellulose synthase and compared to prior data on parallel changes in two other divergent cellulose synthase isomers involved in primary wall synthesis.  The results demonstrate the potential of protein engineering to modulate cellulose synthesis through subtle changes in regulatory regions. (August 2021)

    Figure 1D-F of Zhang et al. 2021 modeling the celluloe microfibril, xyloglucan, and pectin in plant cell wall.

    Zhang et al. 2021 Science

  • A plant cell wall’s unique ability to expand without weakening or breaking—a quality required for plant growth—is due the movement of its cellulose skeleton, according to new research that models the cell wall. The new model, created by Penn State CLSF researchers, reveals that chains of cellulose bundle together within the cell wall, providing strength, and slide against each other when the cell is stretched, providing extensibility. The new study, which appears online May 14 in the journal Science, presents a new concept of the plant cell wall, gives insights into plant cell growth, and could provide inspiration for the design of polymeric materials with new properties. Read PSU news coverage and watch a short video that demostrates the dynamics during stretching.
  • Research led by Enrique Gomez and Esther Gomez at Penn State has identified, for the first time, that cellulose crystals have a preferred orientation relative to the cell wall in plants and may be due to some common consequence of how plants make their cell walls. These findings published in September in Nature Communications may help settle a long-standing debate in the cellulose field — whether crystals within plant cell walls twist — because heaving a preferred orientation suggests that crystals aren’t twisting.  These findings came as a result of applying a technique called grazing-incidence wide-angle X-ray scattering (GIWAXS) “developed for materials science and used extensively for the study of thin films, including polymer films” to the study of plant cell walls. Read PSU news coverage or open access research article in Nature Communications.
  • CLSF researchers determined the structure of a cellulose synthase CesA homotrimer which enables structural insights into the unique nanomachinery used by plants to form cellulose chains and microfibrils from sugar monomers. This structure published in Science (Purushotham et al 2020) provides a detailed entry point for investigating how the enzyme works, how three of the enzymes assemble into trimeric subunits, and how six of the subunits assemble into the cellulose synthesis complex which makes the cellulose microfibril.
  • Our recent manuscript Singh et al. 2020 demonstrates that a full-length plant synthase protein structure can be successfully predicted using computational methods. Our CESA model can be used to explain numerous structure-activity relationships within plant cellulose synthases, and we believe that many researchers will find it useful for the selection and subsequent testing of appropriate mutants in order to optimize biomass properties.
  • The nanoscale architecture of fresh, frozen cell walls from several plants has been imaged using low-temperature scanning electron microscopy (cryo-SEM), revealing the dimensions of the cell wall macrofibrils which are made of cellulose and other components. “Visualising the macrofibrils of several trees and of the model plant Arabidopsis allows us to see how the macrofibril size changes in cell walls with different compositions. This will help us develop models for the content of the macrofibrils ” said Professor Paul Dupree, a co-author of the study in the University of Cambridge and a researcher in CLSF. Read the details in Frontiers of Plant Science at Lyczakowski et al. 2019
  • Using solid-state NMR spectroscopy, Phyo and CLSF researchers showed that low pH neutralizes pectins, which weaken the interactions between pectins and cellulose, which then allow polysaccharide slippage, ultimately leading to wall loosening. These structural and dynamical findings about Arabidopsis primary cell walls have general implications for the mechanism of acid growth of plant cell walls. Read the details in Cellulose at Phyo et al. 2019
  • side view of cellulose model with Glucuronoarabinoxylan

    Fig. 1 Shrestha et al., 2019

    In a collaborative project, Shrestha and colleagues at Oak Ridge National Lab and Penn State, combined experiment and simulation to determine that arabinsose side-chains confer flexibility to xylan, a property that that may explain xylan function in plant cell walls. Read the details at Shrestha et al. 2019 (Arabinose substitution effect on xylan rigidity and self-aggregation).

  • Challenging fundamental cell wall assumptions: an article in the Frontiers in Energy Research Summer 2019 Newsletter entitled Inspiration, Not Imitation: Chemists with Energy Research Centers design molecules for natural function included comments from CLSF’s James Kubicki about honing models on cellulose synthesis and the strength of working in a group of scientists with mixed backgrounds and specialties. Since our group started with a good number of scientist that had never worked on the topic of plant cell walls and continues to add member scientists outside this field, “[we] came in without having the prejudices and biases that people had from reading the literature from the past thirty years… We challenged many of the fundamental assumptions,” Kubicki said, “and one of those was the size of the cellulose microfibril.” Read the full article here
  • Our researchers (Hill et al. 2018) revealed that multiple domains in the enzyme that produces cellulose in secondary cell walls, cellulose synthase (CesA), are involved in protein recognition. Chimeric genes were constructed that swapped domains of the enzyme involved in protein-protein contact, and the ability to recover crystalline cellulose content and stem height in cellulose deficient plants indicated which domains are relevant in the protein-protein recognition or contact.
  • Introduction of a new technique – resonant soft X-ray scattering (RSoXS) – to the study of plant cell walls is reported in a recent paper in Scientific Reports (Ye et al. 2018). RSoXS enhances contrast by tuning the X-ray energy, allowing for the study of cell wall structure on the scale of tens of nanometers. Using this technique, the spacing between microfibrils or microfibril bundles is revealed to be around 20 nm.
  • depiction of arrangements and lengths of cellulose microfibrils (Figure 1 Oehme et al. 2018)A recent paper in the journal Cellulose (Oehme et al. 2018)​ details how researchers from the CLSF have developed computational techniques to gain a greater understanding of how cellulose chains pack together within plant cell walls. Molecular dynamics simulations are combined with quantum mechanical calculations of computational models to produce NMR spectra, which can then be compared to experimental data to validate the different models produced.
  • In an article on “Diffuse Growth of Plant Cell Walls”, Daniel Cosgrove reviews the biophysical basis of cell wall growth, recent paradigm shifts in the role and interactions of cell wall components that compose the cell wall, and insights from atomic force microscopy (AFM) that reveal the details of microfibril organization and motions during wall enlargement.

More information

Publications

  • Roberts, Eric; Yuan, Kai; Chaves, Arielle; Pierce, Ethan; Cresswell, Rosalie; Dupree, Ray; Yu, Xiaolan; Blanton, Richard; Wu, Shu-Zon; Bezanilla; Magdalena; Dupree, Paul; Haigler, Candace; Roberts, Alison. (2024) An alternate route for cellulose microfibril biosynthesis in plants. Science Advances, 10, eadr5188 (2024). DOI: 10.1126/sciadv.adr5188
  • Yu, Jingyi; Del Mundo, Joshua T.; Freychet, Guillaume; Zhernenkov, Mikhail; Schaible, Eric; Gomez, Esther W.; Gomez, Enrique D.; Cosgrove, Daniel J. (2024) Dynamic Structural Change of Plant Epidermal Cell Walls under Strain. Small 2024, 2311832. doi 10.1002/smll.202311832
  • Tryfona, Theodora; Pankratova, Yanina; Petrik, Deborah; Moran, Diego Rebaque; Wightman, Raymond; Yu, Xiaolan; Echevarria-Poza, Alberto; Deralia, Parveen Kumar; Vilaplana, Francisco; Anderson, Charles T.; Hong, Mei; Dupree, Paul (2024) Altering the substitution and cross‐linking of glucuronoarabinoxylans affects cell wall architecture in Brachypodium distachyon. New Phytologist 242(2):524-543. doi 10.1111/nph.19624
  • Ajayi, Oyeyemi; Zelinsky, Ellen; Anderson, Charles T. (2024) A core of cell wall proteins functions in wall integrity responses in Arabidopsis thaliana. Plant Direct. 2024 Apr;8(4):e579. doi: 10.1002/pld3.579

    Cover of Ceullose February 2024 with molecular illustration of CESA embedded in cell membrane bilayer.

    Cover of the February 2024 issue of Cellulose featuring image from Kwansa et al. 2024

  • Kwansa AL, Singh A, Williams JT, Haigler CH, Roberts AW, Yingling YG (2024) Structural determination of a full-length plant cellulose synthase informed by experimental and in silico method. Cellulose 31: 1429-1447. doi 10.1007/s10570-023-05691-x .   This research was highlighted on the cover of the February 2024 issue of Cellulose.
  • Rongpipi, Sintu; William J. Barnes, Oskar Siemianowski, Dan Ye, Joshua T. Del Mundo, Sydney Duncombe, Xiaoran Xin, Chenhui Zhu, Michael F. Toney, Ying Gu, Charles T. Anderson, Enrique D. Gomez, Esther W. Gomez (2024) Matrix polysaccharides affect preferred orientation of cellulose crystals in primary cell walls. Cellulose 31: 1397-1415. doi 10.1007/s10570-023-05702-x
  • Yu, Jingyi; Zhang, Yao; and Cosgrove, Daniel J. (2024). The nonlinear mechanics of highly extensible plant epidermal cell walls. Proceedings of the National Academy of Sciences 121, e2316396121. doi 10.1073/pnas.2316396121
  • Duan, Pu; Hong, Mei (2024) Selective Detection of Intermediate-Amplitude Motion by Solid-State NMR. J. Phys. Chem. B 128: 2293-2303 doi 10.1021/acs.jpcb.3c06839
  • Siemianowski, Oskar; Sintu Rongpipi, Joshua T. Del Mundo, Guillaume Freychet, Mikhail Zhernenkov, Enrique D. Gomez, Esther W. Gomez, Charles T. Anderson (2024) Flexible pectin nanopatterning drives cell wall organization in plants. JACS Au 4 (1): 177-188. doi 10.1021/jacsau.3c00616
  • Cosgrove, D.J. (2024) Structure and growth of plant cell walls. Nat Rev Mol Cell Bio doi 10.1038/s41580-023-00691-y
  • Choi, Juseok; Lee, Jongcheol; Makarem, Mohamadamin; Lee, Chonghan; Lee, Jongcheol; Kiemle, Sarah; Cosgrove, Daniel J; Kim, Seong H. (2023) Tissue-specific directionality of cellulose synthase complex movement inferred from cellulose microfibril polarity in secondary cell walls of Arabidopsis. Scientific Reports 13, Article number: 22007. doi 10.1038/s41598-023-48545-z
  • Gupta, Madhulika; Dupree, Paul; Petridis, Loukas; Smith, Jeremy C. (2023) Patterns in interactions of variably acetylated xylans with hydrophobic cellulose surfaces. Cellulose 30, 11323–11340 (2023). doi 10.1007/s10570-023-05584-z
  • Lee J, Choi J, Feng L, Yu J, Zheng Y, Zhang Q, Lin YT, Sah S, Gu Y, Zhang S, Cosgrove DJ, Seong, SH (2023) Regiospecific Cellulose Orientation and Anisotropic Mechanical Property in Plant Cell Walls. Biomacromolecules 24, 4759-4770. doi 10.1021/acs.biomac.3c00538
  • Rongpipi S, Barnes WJ, Siemianowski O, Del Mundo JT, Wang C, Freychet G, Zhernenkov M, Anderson CT, Gomez EW, Gomez ED. (2023) Measuring calcium content in plants using NEXAFS spectroscopy. Frontiers in Plant Science. 2023 Aug 16; 14:1212126. doi: 10.3389/fpls.2023.1212126
  • Xiang M, Yuan S, Zhang Q, Liu X, Li Q, Leng Z, Sha J, Anderson CT, and Xiao C. (2023). Galactosylation of xyloglucan is essential for the stabilization of the actin cytoskeleton and endomembrane system through the proper assembly of cell walls. J Exp Bot 74, 5104-5123. doi 10.1093/jxb/erad237
  • Lee, Jongcheol, Arielle M. Chaves, Juseok Choi, Alison W. Roberts, and Seong H. Kim.  (2023) Sum frequency generation (SFG) microscopy analysis of cellulose microfibrils in Physcomitrium patens gametophore leaf. Cellulose Jul 7: 1-10. doi 10.1007/s10570-023-05355-w
  • Xin, Xiaoran; Wei, Donghui; Lei, Lei; Zheng, Haiyan; Wallace, Ian; Li, Shundai; Gu, Ying (2023) CALCIUM‐DEPENDENT PROTEIN KINASE32 regulates cellulose biosynthesis through post‐translational modification of cellulose synthase.  New Phytologist 239, 2212-2224.  doi  10.1111/nph.19106
  • Verma, Preeti; Kwansa, Albert L.; Ho, Ruoya; Yingling, Yaroslava G.; Zimmer, Jochen (2023) Insights into substrate coordination and glycosyl transfer of poplar cellulose synthase-8. Structure 31, 1166–1173.  doi 10.1016/j.str.2023.07.010
  • Chae, Inseok; Paniagua-Guerra, Luis E.; Pitcher, Mica L.; Koshani, Roya; Yuan, Mengxue; Lin, Yen-Ting; Lee, Jongcheol; Perini, Steven E.; Sheikhi, Amir; Ramos-Alvarado, Bladimir; Lanagan, Michael T.; Kim, Seong H. (2023) Relaxation dynamics of water in the vicinity of cellulose nanocrystals.  Cellulose 30, 8051-8061. doi 10.1007/s10570-023-05361-y
  • Peng, Xiaopeng; Tong, Botong; Lee, Jongcheol; Wang, Kun; Yu, Xiaojuan; Huang, Xiong; Wen, Jialong; Makarem, Mohamadamin; Pang, Hongying; Hinjan, Subin; Yan, Xiaojing; Yao, Shuangquan; Lu, Fachuang; Wang, Baichen; Peng, Feng; Ralph, John; Kim, Seong H.; Sederoff, Ronald R.; Li, Quanzi (2023) Overexpression of a gibberellin 20-oxidase gene in poplar xylem led to an increase in the size of nanocellulose fibrils and improved paper properties.  Carbohydrate Polymers 314: 120959. doi  10.1016/j.carbpol.2023.120959
  • Wu, Shu-Zon; Chaves, Arielle M.; Li, Rongrong; Roberts, Alison W.; Bezanilla, Magdalena (2023) Cellulose synthase-like D movement in the plasma membrane requires enzymatic activity.  Journal of Cell Biology 222 (6): e202212117.  doi 10.1083/jcb.202212117
  • Del Mundo, Joshua T.; Rongpipi, Sintu; Yang, Hui; Ye, Dan; Kiemle, Sarah N.; Moffitt, Stephanie L.; Troxel, Charles L.; Toney, Michael F.; Zhu, Chenhui; Kubicki, James D.; Cosgrove, Daniel J.; Gomez, Esther W.; Gomez, Enrique D. (2023). Grazing-incidence diffraction reveals cellulose and pectin organization in hydrated plant primary cell wall. Scientific Reports, 13(1), 5421.  doi 10.1038/s41598-023-32505-8
  • Gilcher, E.; Kuch, N.; Del Mundo, J.T.; Ausman, S.; Santiago-Martinez, L.; Clewett, C.; Gomez, E.W.; Gomez, E.D.; Root, T.; Fox, B.; Dumesic, J. (2023) Evolution of the cellulose microfibril through gamma-valerolactone assisted co-solvent and enzymatic hydrolysis. ACS Sustainable Chemistry & Engineering 11 (8): 3270-3283. doi.org/10.1021/acssuschemeng.2c06030
  • Coen, Enrico; Cosgrove, Daniel J. (2023) The mechanics of plant morphogenesis. Science 379, eade8055. doi 10.1126/science.ade8055
  • Tryfona, Theodora; Bourdon, Matthieu; Marques, Rita Delgado; Busse‐Wicher, Marta; Vilaplana, Francisco; Stott, Katherine; Dupree, Paul (2023) Grass xylan structural variation suggests functional specialisation and distinctive interaction with cellulose and lignin. The Plant Journal 113: 1004-1020. 10.1016/j.tcsw.2021.100069
  • Purushotham, Pallinti; Ho, Ruoya; Yu, Long; Fincher, Geoffrey B.; Bulone, Vincent; Zimmer, Jochen (2022) Mechanism of mixed-linkage glucan biosynthesis by barley cellulose synthase–like CslF6 (1,3;1,4)-β-glucan synthase       Science Advances 8(45): eadd1596. doi 10.1126/sciadv.add1596
  • Pfaff, Sarah A.; Wang, Xuan; Wagner, Edward R.; Wilson, Liza A.; Kiemle, Sarah N.; Cosgrove, Daniel J. (2022) Detecting the orientation of newly-deposited crystalline cellulose with fluorescent CBM3.  The Cell Surface 8: 100089.  doi 10.1016/j.tcsw.2022.100089
  • Del Mundo, Joshua T.; Rongpipi, Sintu; Gomez, Enrique D.; Gomez, Esther W. (2022) Characterization of biological materials with soft X-ray scattering. In Small Angle Scattering Part A: Methods for Structural Investigation (Methods in Enzymology), 2022, (ed. J. Tainer), Elsevier.  doi 10.1016/bs.mie.2022.08.042 
  • Rongpipi, Sintu; Del Mundo, Joshua T.; Gomez, Enrique D.; Gomez, Esther W.  (2022) Extracting structural insights from soft X-ray scattering of biological assemblies. In Small Angle Scattering Part B: Methods for Structural Interpretation (Methods in Enzymology), 2022, (ed. J. Tainer), Elsevier.  doi 10.1016/bs.mie.2022.09.017
  • Zhao, Wencheng; Deligey, Fabien; Shekar, Chandra; Mentink-Vigier, Frederic; Wang, Tuo (2022) Current limitations of solid-state NMR in carbohydrate and cell wall research.  Journal of Magnetic Resonance 341: 107263. doi 10.1016/j.jmr.2022.107263
  • Choi, Juseok; Lee, Jongcheol; Makarem, Mohamadamin; Huang, Shixin; Kim, Seong H. (2022) Numerical Simulation of Vibrational Sum Frequency Generation Intensity for Non-Centrosymmetric Domains Interspersed in an Amorphous Matrix: A Case Study for Cellulose in Plant Cell Wall. J. Phys. Chem. B 2022, 126, 35, 6629–6641.   doi  10.1021/acs.jpcb.2c03897
  • Du, Juan; Vandavasi, Venu Gopal; Molloy, Kelly R.; Yang, Hui; Massenburg, Lynnicia N.; Singh, Abhishek; Kwansa, Albert L.; Yingling, Yaroslava G.; O’Neill, Hugh; Chait, Brian T.; Kumar, Manish; Nixon, Tracy (2022) Evidence for Plant-Conserved Region Mediated Trimeric CESAs in Plant Cellulose Synthase Complexes. Biomacromolecules 23(9): 3663-3677.  doi   10.1021/acs.biomac.2c00550
  • Temple, Henry; Phyo, Pyae; Yang, Weibing; Lyczakowski, Jan J.; Echevarría-Poza, Alberto; Yakunin, Igor; Parra-Rojas, Juan Pablo; Terrett, Oliver M.; Saez-Aguayo, Susana; Dupree, Ray; Orellana, Ariel; Hong, Mei; Dupree, Paul (2022) Golgi-localized putative S-adenosyl methionine transporters required for plant cell wall polysaccharide methylation. Nature Plants 8: 656-669. doi 10.1038/s41477-022-01156-4 .   An associated research briefing highlights the Temple et al. 2022 article: “Shutting the door on polysaccharide methylation“. “Identifying potential transporters of SAM into the Golgi is important for understanding the role of pectin modifications in cell wall structure and function”.
  • Cosgrove, Daniel J. (2022) Building an Extensible Cell Wall. Plant Physiology, kiac184.  doi 10.1093/plphys/kiac184
  • Du, Juan; Anderson, Charles T.; Xiao, Chaowen (2022) Dynamics of pectic homogalacturonan in cellular morphogenesis and adhesion, wall integrity sensing and plant development. Nature Plants 8: 332–340  doi 10.1038/s41477-022-01120-2

    Cover art of Biomacromolecules journal artistically representing molecular designs emarging from a flask

    Cover art of the June 13, 2022 issue of Biomacromolecules highlighting Deligey et al. 2022

  • Deligey, Fabien; Frank, Mark A.; Cho, Sung Hyun; Kirui, Alex; Mentink-Vigier, Frederic; Swulius, Matthew T.; Nixon, Tracy; Wang, Tuo (2022) Structure of In Vitro-Synthesized Cellulose Fibrils Viewed by Cryo-Electron Tomography and 13C Natural-Abundance Dynamic Nuclear Polarization Solid-State NMR , Biomacromolecules 23(6): 2290-2301. doi 10.1021/acs.biomac.1c01674 This article was also highlighted on one of the covers of the June 13, 2022 issue of Biomacromolecules.
  • Neuwald, Andrew F.; Yang, Hui; Nixon, Tracy (2022) SPARC: Structural properties associated with residue constraints. Computational and Structural Biotechnology Journal 20: 1702-1715. doi 10.1016/j.csbj.2022.04.005
  • Li, Xingxing; Chaves, Arielle M; Dees, Dianka C.T; Mansoori, Nasim; Yuan, Kai; Speicher, Tori L; Norris, Joanna H; Wallace, Ian S; Trindade, Luisa M; Roberts, Alison W. (2022) Cellulose synthesis complexes are homo-oligomeric and hetero-oligomeric in Physcomitrium patens. Plant Physiology 188: 2115–2130. doi 10.1093/plphys/kiac003
  • Kirui, Alex; Zhao, Wancheng; Deligey, Fabien; Yang, Hui; Kang, Xue; Mentink-Vigier, Frederic; Wang, Tuo (2022)  Carbohydrate-aromatic interface and molecular architecture of lignocellulose.  Nature Communications 13: 538. doi 10.1038/s41467-022-28165-3
  • Chakraborty, Ishita; Rongpipi, Sintu; Govindaraju, Indira; B, Rakesh; Mal, Sib Sankar; Gomez, Esther W.; Gomez, Enrique D.; Kalita, Ranjan Dutta; Nath, Yuthika; Mazumder, Nirmal (2022) An insight into microscopy and analytical techniques for morphological, structural, chemical, and thermal characterization of cellulose. Microscopy Research and Technique, 1-26. doi 10.1002/jemt.24057
  • Cosgrove, Daniel J (2022) Plant Cell Growth and Cell Wall Enlargement. eLS, 1-14. doi 10.1002/9780470015902.a0029421
  • Cheung, Alice Y.; Cosgrove, Daniel J.; Hara-Nishimura, Ikuko; Jürgens, Gerd; Lloyd, Clive; Robinson, David G.; Staehelin, Andrew; Weijers, Dolf (2022) A rich and bountiful harvest: Key discoveries in plant cell biology. The Plant Cell 34: 53-71. doi  10.1093/plcell/koab234

    Image of the supplemental Chemical Reviews cover depicting a cryogenic tank surrounded by colorful models of molecules

    Chemical Reviews May 25 2022 cover

  • Ghassemi, Nader; Poulhazan, Alexandre; Deligey, Fabien; Mentink-Vigier, Frederic; Marcotte, Isabelle; Wang, Tuo (2022) Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants. Chemical Reviews 122: 10036-10086.  doi 10.1021/acs.chemrev.1c00669  This article was featured on the cover of the May 25, 2022 issue of Chemical Reviews (image created by Daniel S. Rouhani)
  • Duncombe, Sydney G; Chethan, Samir G; Anderson, Charles T. (2022) Super-resolution imaging illuminates new dynamic behaviors of cellulose synthase. The Plant Cell 34: 273 – 286. doi 10.1093/plcell/koab227
  • Lin, Wenwei; Tang, Wenxin; Pan, Xue; Huang, Aobo; Gao, Xiuqin; Anderson, Charles T; Yang, Zhenbiao (2021) Arabidopsis pavement cell morphogenesis requires FERONIA binding to pectin for activation of ROP GTPase signaling. Current Biology 32(3): 497-507. doi 10.1016/j.cub.2021.11.030
  • Xue, Jan; Purushotham, Pallinti; Acheson, Justin F; Ho, Ruoya; Zimmer, Jochen; McFarlane, Ciaran; Van Petegem, Filip; Martone, Patrick T; Samuels, Lacey (2021) Functional characterization of a cellulose synthase, CtCESA1, from the marine red alga Calliarthron tuberculosum (Corallinales). Journal of Experimental Botany 73 (3): 680-695. doi 10.1093/jxb/erab414
  • Behar, Hila; Tamura, Kazune; Wagner, Edward R.; Cosgrove, Daniel J.; Brumer, Harry (2021) Conservation of endo-glucanase 16 (EG16) activity across highly divergent plant lineages. Biochemical Journal 478: 3063-3078. doi 10.1042/BCJ20210341
  • Julien, Jeffrey A; Fernandez, Martin G; Brandmier, Katrina M; Del Mundo, Joshua T; Bator, Carol M; Loftus, Lucie A; Gomez, Esther W; Gomez, Enrique D; Glover, Kerney Jebrell (2021) Rapid preparation of nanodiscs for biophysical studies. Archives of Biochemistry and Biophysics 712: 109051. doi 10.1016/j.abb.2021.109051
  • Burris, Jason N; Makarem, Mohamadamin; Slabaugh, Erin; Chaves, Arielle; Pierce, Ethan T; Lee, Jongcheol; Kiemle, Sarah N; Kwansa, Albert L; Singh, Abhishek; Yingling, Yaroslava G; Roberts, Alison W; Kim, Seong H; Haigler, Candace H (2021) Phenotypic effects of changes in the FTVTxK region of an Arabidopsis secondary wall cellulose synthase compared with results from analogous mutations in other isoforms. Plant Direct 5:e335.  doi  10.1002/pld3.335
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    Figure 1B of Purushotham et al., 2020

    Purushotham, Pallinti; Ho, Ruoya; Zimmer, Jochen (2020) Architecture of a catalytically active homotrimeric plant cellulose synthase complex. Science 369(6507): 1089-1094. doi 10.1126/science.abb2978

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    Cover of Cellulose March 2020 depicting figure art from Yang et al., 2020

    Yang, Hui; Kubicki, James D (2020) A density functional theory study on the shape of the primary cellulose microfibril in plants: effects of C6 exocyclic group conformation and H-bonding. Cellulose 27: 2389-2402. doi.org/10.1007/s10570-020-02970-9

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    Journal cover of Cellulose January 2020 showing digital representatons of systems for generating ceuulose microfibrils.

    Images from Huang et al. grace cover of Cellulose January 2020

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  • purple ribbons representing active site of cellulose synthase surround stick molecule on cover of Journal of Physical Chemistry B from Yang et al. 2019 article within

    Art from Yang et al., 2019 is featured on cover of JPC B

    Yang, Hui; McManus, John; Oehme, Daniel; Singh, Abhishek; Yingling, Yaroslava G; Tien, Ming; Kubicki, James D (2019) Simulations of Cellulose Synthesis Initiation and Termination in Bacteria. The Journal of Physical Chemistry Part B 123 (17): 3699–3705. https://doi.org/10.1021/acs.jpcb.9b02433 This article also supplied the cover art for one of two printed issues.

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  • Hill, Joseph L; Josephs, Cooper; Barnes, William J; Anderson, Charles T; Tien, Ming (2018) Longevity in vivo of Primary Cell Wall Cellulose Synthases. Plant Molecular Biology 96(3): 279-289.  https://link.springer.com/article/10.1007%2Fs11103-017-0695-4
  • Scavuzzo-Duggan, Tess R; Chaves, Arielle M; Singh, Abhishek; Sethaphong, Latsavongsakda; Slabaugh, Erin; Yingling, Yaroslava G; Haigler, Candace H; Roberts, Alison W (2018) Cellulose synthase ‘class specific regions’ are intrinsically disordered and functionally undifferentiated.  Journal of Integrative Plant Biology epublished ahead of print. http://onlinelibrary.wiley.com/doi/10.1111/jipb.12637/abstract/

    Cover of Plant Journal Vol 93, Issue 2 showing AFM image of microfibers with colored dots from Zheng et al. 2018

    Image from Zheng et al. 2018 featured on cover of Plant Journal

  • Zheng, Yunzhen; Wang, Xuan; Chen, Yuning; Wagner, Edward; Cosgrove, Daniel J (2018) Xyloglucan in the primary cell wall: assessment by FESEM, selective enzyme digestions and nanogold affinity tags. The Plant Journal 93(2): 211 – 226.  Featured as cover image of Plant Journal Volume 93 Issue 2 and as subject of a research highlight article. http://onlinelibrary.wiley.com/doi/10.1111/tpj.13778/abstract/
  • Barnes, William J; Anderson, Charles T (2018) Release, Recycle, Rebuild: Cell-Wall Remodeling, Autodegradation, and Sugar Salvage for New Wall Biosynthesis during Plant Development. Molecular Plant 11(1): 31-46. https://doi.org/10.1016/j.molp.2017.08.011
  • Tran, Mai L; McCarthy, Thomas W; Sun, Hao; Wu, Shu-Zon; Norris, Joanna H; Bezanilla, Magdalena; Vidali, Luis; Anderson, Charles T; Roberts, Alison W (2018) Direct observation of the effects of cellulose synthesis inhibitors using live cell imaging of Cellulose Synthase (CESA) in Physcomitrella patens. Scientific Reports 8(1):735.  https://www.nature.com/articles/s41598-017-18994-4
  • Cosgrove, Daniel J (2018) Diffuse growth of plant cell walls. Plant Physiology pp.01541.2017. http://www.plantphysiol.org/content/176/1/16
  • Makarem, Mohamadamin; Lee, Christopher M; Sawada, Daisuke; O’Neill, Hugh M; Kim, Seong H (2018) Distinguishing Surface versus Bulk Hydroxyl Groups of Cellulose Nanocrystals Using Vibrational Sum Frequency Generation Spectroscopy. The Journal of Physical Chemistry Letters 9(1): 70-75. http://dx.doi.org/10.1021/acs.jpclett.7b02729
  • Yang, Hui; Wang, Tuo; Oehme, Daniel; Petridis, Loukas; Hong, Mei; Kubicki, James D (2018) Structural factors affecting 13C NMR chemical shifts of cellulose: a computational study. Cellulose 25(1): 23-36.  https://link.springer.com/article/10.1007%2Fs10570-017-1549-6
  • Phyo, Pyae; Wang, Tuo; Kiemle, Sarah N; O’Neill, Hugh; Pingali, Sai Venkatesh; Hong, Mei; Cosgrove, Daniel J (2017) Gradients in wall mechanics and polysaccharides along growing inflorescence stems. Plant Physiology 175(4): 1593-1607.  http://www.plantphysiol.org/content/175/4/1593
  • Durachko, Daniel M; Park, Yong Bum; Zhang, Tian ; Cosgrove, Daniel J (2017) Biomechanical Characterization of Onion Epidermal Cell Walls, BIO-PROTOCOL 7(24): e2662. https://bio-protocol.org/e2662
  • Zhang, Tian; Cosgrove, Daniel J (2017) Preparation of Onion Epidermal Cell Walls for Imaging by Atomic Force Microscopy (AFM). BIO-PROTOCOL 7(24): e2647. https://bio-protocol.org/e2647
  • O’Neill, Hugh; Pingali, Sai Venkatesh; Petridis, Loukas; He, Junhong; Mamontov, Eugene; Hong, Liang; Urban, Volker; Evans, Barbara; Langan, Paul; Smith, Jeremy C; Davison, Brian H (2017) Dynamics of water bound to crystalline cellulose. Scientific Reports 7: 11840.  https://www.nature.com/articles/s41598-017-12035-w
  • Rui, Yue; Xiao, Chaowen; Yi, Hojae; Kandemir, Baris; Wang, James Z; Puri, Virendra M; Anderson, Charles T (2017) POLYGALACTURONASE INVOLVED IN EXPANSION3 Functions in Seedling Development, Rosette Growth, and Stomatal Dynamics in Arabidopsis thaliana. The Plant Cell 29: 2413-2432. http://dx.doi.org/10.1105/tpc.17.00568
  • Phyo, Pyae; Wang, Tuo; Xiao, Chaowen; Anderson, Charles T; Hong, Mei (2017) Effects of Pectin Molecular Weight Changes on the Structure, Dynamics, and Polysaccharide Interactions of Primary Cell Walls of Arabidopsis thaliana: Insights from Solid-State NMR. Biomacromolecules 18(9): 2937-2950.  http://dx.doi.org/10.1021/acs.biomac.7b00888
  • Norris, Joanna H; Li, Xingxing; Huang, Shixin; Van de Meene, Allison ML; Tran, Mai L; Killeavy, Erin; Chaves, Arielle M; Mallon, Bailey; Mercure, Danielle; Tan, Hwei-Ting; Burton, Rachel A; Doblin, Monika S; Kim, Seong H; Roberts, Alison W (2017) Functional specialization of cellulose synthase isoforms in a moss shows parallels with seed plants. Plant Physiology 175: 210-222. https://doi.org/10.1104/pp.17.00885
  • Cho, Sung Hyun; Purushotham, Pallinti;Fang, Chao; Maranas, Cassandra; Díaz-Moreno, Sara M; Bulone, Vincent; Zimmer, Jochen; Kumar, Manish; Nixon, B. Tracy (2017) Synthesis and Self-Assembly of Cellulose Microfibrils from Reconstituted Cellulose Synthase. Plant Physiology 175: 146-156.  http://dx.doi.org/10.1104/pp.17.00619
  • Zheng, YunZhen; Cosgrove, Daniel J; Ning, Gang (2017) High-Resolution Field Emission Scanning Electron Microscopy (FESEM) Imaging of Cellulose Microfibril Organization in Plant Primary Cell Walls. Microscopy and Microanalysis: 1-7.   https://doi.org/10.1017/S143192761701251X
  • Cosgrove, Daniel J (2017) Microbial Expansins. Annu Rev Microbiol 71: 479-497  http://dx.doi.org/10.1146/annurev-micro-090816-093315
  • Chen, Xing; Lee, Christopher M; Wang, Hong-Fei; Lasse, Jensen; Kim, Seong H (2017) Vibrational Sum-Frequency-Generation (SFG) Spectroscopy of Bulk Materials Consisting of SFG active Nanocrystals: Experimental and Theoretical Study of Azimuth Angle and Polarization Dependences of SFG Spectral Features of Uniaxially-Aligned Cellulose Crystals. The Journal of Physical Chemistry Part C 121(34): 18876-18886. http://dx.doi.org/10.1021/acs.jpcc.7b03037
  • Kafle, Kabindra; Yong Bum, Park; Lee, Christoper M; Stapleton, Joshua J; Kiemle, Sarah N; Cosgrove, Daniel J; Kim, Seong H (2017) Effects of mechanical stretching on average orientation of cellulose and pectin in onion epidermis cell wall: A polarized FT-IR study. Cellulose 24: 3145-3154.  http://dx.doi.org/10.1007/s10570-017-1337-3
  • Goodell; Barry, Zhu, Yuan; Kim, Seong H; Kafle, Kabindra; Eastwood, Daniel; Daniel, Geoffrey; Jellison, Jody; Yoshida, Makoto; Groom, Leslie; Pingali, Sai Venkatesh; O’Neill, Hugh (2017) Modification of the nanostructure of lignocellulose cell walls via a non-enzymatic lignocellulose deconstruction system in brown rot wood-decay fungi. Biotechnology for Biofuels 10:179. https://doi.org/10.1186/s13068-017-0865-2
  • Ma, Ding; Lee, Christoper M; Chen, Yizhu; Mehta, Nikhil; Kim, Seong H; Liu, Zhiwen (2017) Vibrational sum frequency generation digital holography. Applied Physics Letters 110: 251601. https://doi.org/10.1063/1.4986451 Selected as an Editor’s Pick!
  • Zhang, Tian; Vavylonis, Dimitrios; Durachko, Daniel M; Cosgrove, Daniel J (2017) Nanoscale Movements of Cellulose Microfibrils in Primary Cell Walls. Nature Plants 3: 17056  http://dx.doi.org/10.1038/nplants.2017.56
  • Barnes, William J; and Anderson Charles T (2017) Acetyl Bromide Soluble Lignin (ABSL) Assay for Total Lignin Quantification from Plant Biomass. Bio-protocol 2017 7(5): e2149. DOI: 10.21769/BioProtoc.2149
  • Makarem, Mohamadamin; Sawada, Daisuke; O’Neill, Hugh M; Lee, Christopher M; Kafle, Kabindra; Park, Yong Bum; Mittal, Ashutosh; Kim, Seong H (2017) Dependence of Sum Frequency Generation (SFG) Spectral Features on the Mesoscale Arrangement of SFG-Active Crystalline Domains Interspersed in SFG-Inactive Matrix: A Case Study with Cellulose in Uniaxially Aligned Control Samples and Alkali-Treated Secondary Cell Walls in Plants. J. Phys. Chem. C 121(18): 10249-10257. DOI: 10.1021/acs.jpcc.7b02770
  • Chae, Inseok; Ahmed, Saad; Atitallah, Hassene Ben; Luo, Jiawei; Wang, Qing; Ounaies, Zoubeida; Kim, Seong H (2017) Vibrational Sum Frequency Generation (SFG) Analysis of Ferroelectric Response of PVDF-Based Copolymer and Terpolymer. Macromolecules 50(7): 2838-2844.  http://dx.doi.org/10.1021/acs.macromol.7b00188
  • Kim, Hee Jin; Lee, Christopher M; Dazen, Kevin; Delhom, Christopher D; Liu, Yongliang; Rodgers, James E; French, Alfred D; Kim, Seong H (2017) Comparative physical and chemical analyses of cotton fibers from two near isogenic upland lines differing in fiber wall thickness. Cellulose 24: 2385–2401.   DOI 10.1007/s10570-017-1282-1
  • Zamil, M Shafayet; Yi, Hojae; Puri, Virendra M (2017) A multiscale FEA framework for bridging cell-wall to tissue-scale mechanical properties: the contributions of middle lamella interface and cell shape. Journal of Materials Science 52 (13): 7947–7968. DOI: 10.1007/s10853-017-0999-4
  • Marek, Antonin; Voinov, Maxim A; Smirnov, Alex I (2017) Spin Probe Multi-Frequency EPR Study of Unprocessed Cotton Fibers. Cell Biochem Biophys 75: 211.  DOI 10.1007/s12013-017-0787-4
  • Xiao, Chaowen; Barnes, William J; Zamil, M Shafayet; Yi, Hojae; Puri, Virendra M; Anderson, Charles T (2017) Activation tagging of Arabidopsis POLYGALACTURONASE INVOLVED IN EXPANSION2 promotes hypocotyl elongation, leaf expansion, stem lignification, mechanical stiffening, and lodging. Plant Journal 89:   1159–1173 . DOI: http://onlinelibrary.wiley.com/doi/10.1111/tpj.13453/epdf
  • Basu, Snehasish, Omadjela, Okako; Zimmer, Jochen; Catchmark, Jeffrey M (2017) Impact of plant matrix polysaccharides on cellulose produced by surface-tethered cellulose synthases.  Carbohydrate Polymers 162 (2017) 93–99   DOI: 10.1016/j.carbpol.2017.01.005
  • Lee, Christopher M; Chen, Xing; Weiss, Philip A; Jensen, Lasse; Kim, Seong H (2017) Quantum Mechanical Calculations of Vibrational Sum-Frequency-Generation (SFG) Spectra of Cellulose: Dependence of the CH and OH Peak Intensity on the Polarity of Cellulose Chains within the SFG Coherence Domain.  The Journal of Physical Chemistry Letters 8: 55-60.   DOI: 10.1021/acs.jpclett.6b02624
  • Vandavasi, Vandavasi Gopal; and O’Neill, Hugh (2016) Heterologous Expression and Purification of Catalytic Domain of CESA1 from Arabidopsis. Bio-protocol 6(20): e1965. DOI: 10.21769/BioProtoc.1965
  • Wang, Bo; McClosky, Daniel D; Anderson, Charles T; Gong, Chen (2016) Synthesis of a suite of click-compatible sugar analogs for probing carbohydrate metabolism.  Carbohydrate Research 433: 54-62.  DOI: 10.1016/j.carres.2016.07.012
  • Xiao, Chaowen; and Anderson, Charles T (2016) Interconnections between cell wall polymers, wall mechanics, and cortical microtubules: Teasing out causes and consequences.  Article Addendum in Plant Signaling & Behavior 11(9): e1215396.  DOI:  10.1080/15592324.2016.1215396
  • Wang, Tuo; Chen, Yuning; Tabuchi, Akira; Hong, Mei; and Cosgrove, Daniel (2016) The Target of β-Expansin EXPB1 in Maize Cell Walls from Binding and Solid-State NMR Studies. Plant Physiol. online ahead of print. DOI: 10.1104/pp.16.01311
  • Pandey, Jyotsna L.; Kiemle, Sarah N; Richard, Tom L.; Zhu, Yimin; Cosgrove, Daniel; and Anderson, Charles T. (2016) Investigating Biochemical and Developmental Dependencies of Lignification with a Click-Compatible Monolignol Analog in Arabidopsis thaliana Stems. Front. Plant Sci., online ahead of print. DOI: 10.3389/fpls.2016.01309
  • Li, Shundai; Bashline, Logan; Zheng, Yunzhen; Xin, Xiaoran; Huang, Shixin; Kong, Zhaosheng; Kim, Seong H; Cosgrove, Daniel; and Gu, Ying (2016) Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants. Proc. Natl. Acad. Sci. U. S. A. 113(40): 11348-11353 DOI: 10.1073/pnas.1613273113
  • Purushotham, Pallinti; Cho, Sung Hyun; Díaz-Moreno, Sara M; Kumar, Manish; Nixon, B. Tracy; Bulone, Vincent; and Zimmer, Jochen (2016) A single heterologously expressed plant cellulose synthase isoform is sufficient for cellulose microfibril formation in vitro. Proc. Natl. Acad. Sci. U. S. A. 113(4): 11360-11365 DOI: 10.1073/pnas.1606210113
  • Wang, Tuo; Phyo, Pyae; and Hong, Mei (2016) Multidimensional solid-state NMR spectroscopy of plant cell walls. Solid State Nucl. Magn. Reson. 78: 56-63. DOI: 10.1016/j.ssnmr.2016.08.001
  • Ogawa, Yu; Lee, Christopher M; Nishiyama, Yoshiharu; and Kim, Seong H (2016) Absence of Sum Frequency Generation in Support of Orthorhombic Symmetry of α-Chitin. Macromolecules 49(18): 7025-7031. DOI: 10.1021/acs.macromol.6b01583
  • Haigler, Candace H.; Davis, Jonathan K.; Slabaugh, Erin; and Kubicki, James D (2016) Biosynthesis and assembly of cellulose. Molecular Cell Biology of the Growth and Differentiation of Plant Cells, online ahead of print. DOI: 10.1201/b20316
  • Nixon, B. Tracy; Mansouri, Katayoun; Singh, Abhishek; Du, Juan; Davis, Jonathan K.; Lee, Jung-Goo; Slabaugh, Erin; Vandavasi, Venu Gopal; O’Neill, Hugh; Roberts, Eric M.; Roberts, Alison W.; Yingling, Yaroslava G.; and Haigler, Candace H. (2016) Comparative Structural and Computational Analysis Supports Eighteen Cellulose Synthases in the Plant Cellulose Synthesis Complex. Sci. Rep. 6: 28696. DOI: 10.1038/srep28696
  • Du, Juan; Vepachedu, Venkata; Cho, Sung Hyun; Kumar, Manish; and Nixon, B. Tracy (2016) Structure of the Cellulose Synthase Complex of Gluconacetobacter hansenii at 23.4 Å Resolution. PLoS One 11(5): e0155886. DOI: 10.1371/journal.pone.0155886
  • Wang, Tuo; Yang, Hui; Kubicki, James D; and Hong, Mei (2016) Cellulose Structural Polymorphism in Plant Primary Cell Walls Investigated by High-Field 2D Solid-State NMR spectroscopy and Density Functional Theory Calculations. Biomacromolecules 17: 2210-2222. DOI: 10.1021/acs.biomac.6b00441
  • Slabaugh, Erin; Scavuzzo-Duggan, Tess R; Chaves, Arielle M; Wilson, Liza; Wilson, Carmen; Davis, Jonathan K; Cosgrove, Daniel J; Anderson, Charles T; Roberts, Alison W; and Haigler, Candace H (2016) The valine and lysine residues in the conserved FxVTxK motif are important for the function of phylogenetically distant plant cellulose synthases. Glycobiology 26(5): 509-519. DOI: 10.1093/glycob/cwv118
  • Tran, Mai L; and Roberts, Alison W (2015) Cellulose synthase (CESA) gene expression profiling of Physcomitrella patens. Plant Biol. (Berlin, Ger.) 18(3): 363-368. DOI: 10.1111/plb.12416
  • McClosky, Daniel D; Wang, Bo; Chen, Gong; and Anderson, Charles T. (2016) The click-compatible sugar 6-deoxy-alkynyl glucose metabolically incorporates into Arabidopsis root hair tips and arrests their growth. Phytochemistry (Elsevier) 123: 16-24. DOI: 10.1016/j.phytochem.2016.01.007
  • Berry, Elizabeth A; Tran, Mai L; Dimos, Christos S; Budziszek, Michael J; Scavuzzo-Duggan, Tess R; and Roberts, Alison W. (2016) Immuno and affinity cytochemical analysis of cell wall composition in the moss Physcomitrella patens. Front. Plant Sci. 7: 248. DOI: 10.3389/fpls.2016.00248

    Cover of the journal Cellulose from Feburary 2016 showing image of molecular model from Sethaphong et al. 2016

    Molecular models from Sethaphong et al. 2016 featured on cover of Cellulose (February 2016)

  • Rui, Yue; and Anderson, Charles T (2016) Functional analysis of cellulose and xyloglucan in the walls of stomatal guard cells of Arabidopsis thaliana. Plant Physiol. 170: 1398-1419. DOI: 10.1104/pp.15.01066
  • Sethaphong, Latsavongsakda; Davis, Jonathan K; Slabaugh, Erin; Singh, Abhishek; Haigler, Candace H; and Yingling, Yaroslava G (2016) Prediction of the structures of the plant-specific regions of vascular plant cellulose synthases and correlated functional analysis. Cellulose 23(1): 145-161. DOI: 10.1007/s10570-015-0789-6 In addition, their image was chosen for the cover of the February 2016 issue of Cellulose
  • Nagachar, Nivedita; and McManus, John B (2016) Microbial Cellulose Synthesis. Microbial Factories: Biofuels, Waste Treatment 1: 203-216. DOI: 10.1007/978-81-322-2598-0
  • Basu, Snehasish; Omadjela, Okako; Gaddes, David; Tadigadapa, Srinivas; Zimmer, Jochen; and Catchmark, Jeffrey M (2016) Cellulose Microfibril Formation by Surface-Tethered Cellulose Synthase Enzymes. ACS Nano 10: 1896-1907. DOI: 10.1021/acsnano.5b05648
  • Atomic force microscopy images overlaid on lower magnification cells separated by walls on the cover of the January 2016 issue of The Plant Journal

    Cover of The Plant Journal, January 2016

    Cosgrove, Daniel (2016) Catalysts of plant cell wall loosening (2016) F1000Research 5: 119. DOI: 10.12688/f1000research.7180.1

  • Zhang, Tian; Zheng, Yunzhen; and Cosgrove, Daniel J (2016) Spatial Organization of Cellulose Microfibrils and Matrix Polysaccharides in Primary Plant Cell Walls as Imaged by Multi-Channel Atomic Force Microscopy. Plant J. 85(2): 179-192. DOI: 10.1111/tpj.13102 In addition, their image was chosen for the cover of the January 2016 issue of The Plant Journal.
  • Lee, Christopher M; Kafle, Kabindra; Huang, Shixin; and Kim, Seong H (2016) Multimodal Broadband Vibrational Sum Frequency Generation (MM-BB-V-SFG) Spectrometer and Microscope. J. Phys. Chem. B 120(1): 102-116. DOI: 10.1021/acs.jpcb.5b10290
  • Cosgrove, Daniel J (2016) Plant cell wall extensibility: connecting plant cell growth with cell wall structure, mechanics, and the action of wallmodifying enzymes. J. Exp. Bot. 67(2): 463-476 (2016). DOI: 10.1093/jxb/erv511
  • Anderson, Charles T (2016) We be jammin’: an update on pectin biosynthesis, trafficking and dynamics. J. Exp. Bot. 67(2): 495-502. DOI: 10.1093/jxb/erv501
  • Wang, Tuo; and Hong, Mei (2016) Solid-state NMR investigations of cellulose structure and interactions with matrix polysaccharides in plant primary cell walls. J. Exp. Bot. 67(2): 503-514. DOI: 10.1093/jxb/erv416
  • Vandavasi, Venu Gopal; Putnam, Daniel K; Zhang, Qiu; Petridis, Loukas; Heller, William T; Nixon, B. Tracy; Haigler, Candace H; Kalluri, Udaya; Coates, Leighton; Langan, Paul; Smith, Jeremy C; Meiler, Jens; and O’Neill, Hugh A (2016) Structural Study of CESA1 catalytic domain of Arabidopsis thaliana Cellulose Synthesis Complex: Evidence for CESA trimers. Plant Physiol. 170: 123-135. DOI: 10.​1104/​pp.​15.​01356Cover of Plant Physiology depicting artistic representation of cellulose synthase complex on cell membrane and cellulose fiber strands bundling on other side of bilayer member In addition, their image was chosen for the cover of the January 2016 issue of Plant Physiology.
  • McManus, John B; Deng, Ying; Nagachar, Nivedita; Kao, Teh-hui; and Tien, Ming (2016) AcsA–AcsB: The core of the cellulose synthase complex from Gluconacetobacter hansenii ATCC23769. Enzyme Microb. Technol. 82: 58-65. DOI: 10.1016/j.enzmictec.2015.08.015
  • Xiao, Chaowen; Zhang, Tian; Zheng, Yunzhen; Cosgrove, Daniel J; and Anderson, Charles T (2016) Xyloglucan deficiency disrupts microtubule stability and cellulose biosynthesis in Arabidopsis, altering cell growth and morphogenesis. Plant Physiol. 170(1): 234-249. DOI: 10.1104/pp.15.01395
  • Dumont, Marie; Lehner, Arnaud; Bardor, Muriel; Burel, Carole; Vauzeilles, Boris; Lerouxel, Olivier; Anderson, Charles T; Mollet, Jean-Claude; and Lerouge, Patrice (2015) Inhibition of fucosylation of cell wall components by 2-fluoro 2-deoxy-L-fucose induces defects in root cell elongation. Plant J. 84(6): 1137-1151. DOI: 10.1111/tpj.13071
  • Lee, Christopher M; Kubicki, James D; Fan, Bingxin; Zhong, Linghao; Jarvis, Michael C; and Kim, Seong H (2015) Hydrogen-Bonding Network and OH Stretch Vibration of Cellulose: Comparison of Computational Modeling with Polarized IR and SFG Spectra. J. Phys. Chem. B 119(49): 15138-15149. DOI: 10.1021/acs.jpcb.5b08015
  • Li, Shundai; Lei, Lei; Yingling, Yaroslava G; and Gu, Ying (2015) Microtubules and cellulose biosynthesis: the emergence of new players. Curr. Opin. Plant Biol. 28: 76-82. DOI: 10.1016/j.pbi.2015.09.002
  • Images of gametophyte and sporophyte phases of moss featured on Cellulose December 2015 journal cover

    Dec. 2015 Cellulose journal cover

    Park, Yong Bum; Kafle, Kabindra; Lee, Christopher M; Cosgrove, Daniel J; and Kim, Seong H (2015) Does cellulose II exist in native alga cell walls? Cellulose structure of Derbesia cell walls studied with SFG, IR and XRD. Cellulose 22(6): 3531-3540. DOI: 10.1007/s10570-015-0750-8 In addition, their image was chosen for the cover of the December 2015 issue of Cellulose.

  • Erbakan, Mustafa; Curtis, Brandon S; Nixon, B. Tracy; Kumar, Manish; and Curtis, Wayne R (2015) Advancing Rhodobacter sphaeroides as a platform for expression of functional membrane proteins. Protein Expression Purif. 8(7): 1011-1023. DOI: 10.1016/j.pep.2015.05.012
  • Lee, Christopher M; Gu, Jin; Kafle, Kabindra; Catchmark, Jeffrey M; and Kim, Seong H (2015) Cellulose produced by Gluconacetobacter xylinus strains ATCC 53524 and ATCC 23768: pellicle formation, post-synthesis aggregation and fiber density. Carbohydr. Polym. 133: 270-276. DOI: 10.1016/j.carbpol.2015.06.091
  • Bashline, Logan; Li, Shundai; Zhu, Xiaoyu; and Gu, Ying (2015) The TWD40-2 protein and the AP2 complex cooperate in the clathrin-mediated endocytosis of cellulose synthase to regulate cellulose biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 112(41): 12870-12875. DOI: 10.1073/pnas.1509292112
  • Kafle, Kabindra; Shin, H; Lee, Christopher M; Park, Sunkyu; and Kim, Seong H (2015) Progressive structural changes of Avicel, bleached softwood, and bacterial cellulose during enzymatic hydrolysis. Sci. Rep. 5: 15102. DOI:10.1038/srep15102
  • Lei, Lei; Singh, Abhishek; Bashline, Logan; Li, Shundai; Yingling, Yaroslava G; and Gu, Ying (2015) CELLULOSE SYNTHASE INTERACTIVE1 Is Required for Fast Recycling of Cellulose Synthase Complexes to the Plasma Membrane in Arabidopsis. Plant Cell 27(10): 2926-2940. DOI: 10.​1105/​tpc.​15.​00442
  • Zamil, M. Shafayet; Yi, Hojae; and Puri, Virendra M (2015)The mechanical properties of plant cell walls soft material at the subcellular scale: the implications of water and of the intercellular boundaries. J. Mater. Sci. 50(20): 6608-6623. DOI: 10.1007/s10853-015-9204-9
  • Bi, Yunchen; Hubbard, Caitlin; Purushotham, Pallinti; and Zimmer, Jochen (2015) Insights into the structure and function of membrane-integrated processive glycosyltransferases. Curr. Opin. Struct. Biol. 34: 78-86. DOI: 10.1016/j.sbi.2015.07.008
  • Cho, Sung Hyun; Du, Juan; Sines, Ian; Poosarla, Venkata Giridhar; Vepachedu, Venkata; Kafle, Kabindra; Park, Yong Bum; Kim, Seong H; Kumar, Manish; and Nixon, B. Tracy (2015) In vitro synthesis of cellulose microfibrils by membrane protein from protoplasts of the non-vascular plant Physcomitrella patens. Biochem. J. 470(2): 195-205. DOI: 10.1042/BJ20141391
  • Lee, Christopher M; Dazen, Kevin; Kafle, Kabindra; Moore, Andrew; Johnson, David K; Park, Sunkyu; and Kim, Seong H (2015) Correlations of Apparent Cellulose Crystallinity Determined by XRD, NMR, IR, Raman, and SFG Methods. Adv. Polym. Sci., 1-17. DOI: 10.1007/12_2015_320
  • Erbakan, Mustafa; Curtis, Brandon S; Nixon, B. Tracy; Kumar, Manish; and Curtis, Wayne R (2015) Advancing Rhodobacter sphaeroides as a platform for expression of functional membrane proteins. Protein Expression Purif. 8(7): 1011-1023. DOI: 10.1016/j.pep.2015.05.012
  • Wang, Tuo; Park, Yong Bum; Cosgrove, Daniel; and Hong, Mei (2015) Cellulose-Pectin Spatial Contacts Are Inherent to Never-Dried Arabidopsis thaliana Primary Cell Walls: Evidence from Solid-State NMR. Plant Physiol. 168 (3): 871-884. DOI: 10.1104/pp.15.00665
  • Kong, Zhaosheng; Ioki, Motohide; Braybrook, Siobhan; Li, Shundai; Ye, Zheng-Ha; Lee, Yuh-Ru Julie; Hotta, Takashi; Chang, Anny; Tian, Juan; Wang, Guangda; and Liu, Bo (2015) Kinesin-4 functions in vesicular transport on cortical microtubules and regulates cell wall mechanics during cell elongation in plants. Mol. Plant 8(7): 1011-1023. DOI: 10.1016/j.molp.2015.01.004
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